il17a  (R&D Systems)

Journal: Journal of Animal Science and Biotechnology

Article Title: Baicalin alleviates mastitis in dairy cows by targeting IL-17RA to inhibit IL-17 signaling pathway activation

doi: 10.1186/s40104-026-01401-2

Figure Lengend Snippet: Schematic overview of baicalin's mechanism in alleviating mastitis by regulating the IL-17RA-mediated IL-17 signaling pathway. A Oral administration of baicalin to mice and dairy cows. B In vivo release of LPS by E. coli . C LPS activated the TLR4/MyD88/NF-κB pathway in epithelial cells. D NF-κB was activated within the cell nucleus. E E. coli infection increases IL-17A content in mammary glands. F IL-17A binds to IL-17RA on the cell membrane surface, thereby activating the IL-17 signaling pathway. G Activation of the MAPK signaling pathway and ERK signaling pathway. H Production of IL-6, TNFα and IL-1β. I TNFα activated TNF signaling pathway. J TJ structure damage. K Baicalin inhibits IL-17RA signal transduction, thereby preventing activation of the IL-17 signaling pathway

Article Snippet: An in vitro mastitis model was established by treating cells with 5 μg/mL LPS (Sigma, USA) for 12 h. Baicalin (purity ≥ 95%) was purchased from Macklin (Shanghai, China), with a concentration of 20 μmol/L for 24 h. Furthermore, the TNFα inhibitor SPD304 and recombinant IL-17A (rIL-17A) were purchased from MedChemExpress (MCE, USA) and were used at concentrations of 5 μmol/L (for 2 h) and 100 ng/mL (for 12 h), respectively.

Techniques: In Vivo, Infection, Membrane, Activation Assay, Transduction